Dr Lynn Paterson

 

I joined Heriot-Watt University in July 2007 as an RCUK Academic Fellow, having previously worked within the Optical Trapping Group at the University of St Andrews. My research falls under the broad theme of biophotonics. I use optical manipulation (optical tweezers/optical trap) for studies into the life sciences.

 

Research

A laser beam, focused tightly through a microscope objective can be used as a tool to pick up and move microscopic objects such as cells or intracellular objects. Using a different wavelength or a different mode, the focused beam can be used as a microdissection tool. This allows us to control the complicated biochemistry of a cell simply by exerting forces on a cell or selectively destroying parts of the cell. Foreign point detectors (functionalised microspheres or nanoparticles) can also be placed inside cells and manipulated internally, using merely laser light. A. Silica microspheres are attached to the surface of HL60 cells (human promyelocytic leukaemia cell line) to assist with the all-optical sorting of the sphere-labelled cells from unlabelled cells (using spheres coated in antibodies to the cells’ surface antigens). Silica microspheres have a higher refractive index than the cells so have an enhanced response to the optical sorting landscape. B. A violet diode laser (wavelength 405nm), focused to a spot size of about one micrometer diameter, is used to change the permeability of the plasma membrane of CHO cells (Chinese hamster ovary fibroblasts), allowing the cell to uptake material that is usually non-membrane permeable. C. Erythrocytes and lymphocytes can be separated in the circularly symmetrical optical potential landscape of a Bessel beam due to their difference in size. Spherical lymphocytes are drawn to the beam centre where the intensity is highest, but bi-concave disc shaped erythrocytes are trapped in the outer rings of the beam. The beam is propagation invariant and cells in the central maximum can be guided vertically by the radiation pressure from the beam. D. A Q-switched, frequency doubled Nd:YAG (wavelength 523nm) beam, focused down to a 500nm diameter spot, cuts a Giemsa stained, metaphase MJ chromosome (Muntjac fibroblast). The DNA from microdissected fragments like these are used as templates for PCR to create FISH probes for chromatid break studies.

I am alwaysinterested to hear from anyone who is intersted in carrying out a PhD in this area or anyone would like to come and work in the optical manipulation and biophotonics lab or simply pay us a visit. 

 

Publications

Cell sorting using a circularly symmetrical beam.

L. Paterson, E. Papagiakoumou, G. Milne, V. Garcés-Chávez, T. Briscoe, W. Sibbett, K. Dholakia and A.C. Riches. 2007. Passive optical separation within a ‘non-diffracting’ light beam. Journal of Biomedical Optics 12, article number 054017. Paterson, L., E. Papagiakoumou, G. Milne, V. Garcés-Chávez, S. A. Tatarkova, W. Sibbett, F. J. Gunn-Moore, P. E. Bryant, A. C. Riches & K. Dholakia. 2005. Light-induced cell separation in a tailored optical landscape. Applied Physics Letters 87, article number 123901. 

Laser assisted injection into cells (photoporation)

Paterson, L., B. Agate, M. Comrie, R. Ferguson, T.K. Lake, J.E. Morris, A.E. Carruthers, C.T.A. Brown, W. Sibbett, P.E. Bryant, F. Gunn-Moore, A.C. Riches & K. Dholakia. 2005. Photoporation and cell transfection using a violet diode laser. Optics Express 13: 595-600. 

Rotating optically trapped particles

MacDonald, M.P., L. Paterson, K. Volke-Sepulveda, J. Arlt, W. Sibbett & K. Dholakia. 2002. Creation and manipulation of three-dimensional optically trapped structures. Science 296: 1101-1103. 

  

Full list of publications

2007

K. Dholakia, W. M. Lee, L. Paterson, M. P. MacDonald, R. McDonald, I. Andreev, P. Mthunzi, C. T. A. Brown, R. F. Marchington & A. C. Riches. 2007. Optical Separation of Cells on Potential Energy Landscapes: Enhancement with Dielectric Tagging. IEEE Journal of selected topics in Quantum Electronics 13: 1646-1654. [abstract] [full text] Paterson, L., E. Papagiakoumou, G. Milne, V. Garces-Chavez, T. Briscoe, W. Sibbett, K. Dholakia, & A. C. Riches. 2007. Passive optical separation within a 'nondiffracting' light beam. Journal of Biomedical Optics 12: Art. No. 054017. [abstract] [full text (limited access) ] Fischer P, A. McWilliam, L. Paterson, C. T. A. Brown, W. Sibbett, K. Dholakia & M. P. MacDonald. 2007. Two-photon ablation with 1278 nm laser radiation. Journal of Optics A- Pure and Applied Optics 9: S19-S23. [abstract] [full text] 

2006

Jess, P.R.T., V. Garcés-Chávez, D. Smith, M. Mazilu, L. Paterson, A. Riches, C. S. Herrington, W. Sibbett, & K. Dholakia. 2006. Dual beam fibre trap for Raman micro-spectroscopy of single cells. Optics Express 14: 5779-5791. [link] 

2005

Paterson, L., E. Papagiakoumou, G. Milne, V. Garcés-Chávez, S. A. Tatarkova, W. Sibbett, F. J. Gunn-Moore, P. E. Bryant, A. C. Riches & K. Dholakia. 2005. Light-induced cell separation in a tailored optical landscape. Applied Physics Letters 87, article number 123901. [abstract] [full text] Paterson, L., B. Agate, M. Comrie, R. Ferguson, T.K. Lake, J.E. Morris, A.E. Carruthers, C.T.A. Brown, W. Sibbett, P.E. Bryant, F. Gunn-Moore, A.C. Riches & K. Dholakia. 2005. Photoporation and cell transfection using a violet diode laser. Optics Express 13: 595-600. [link] 

2004

MacDonald, M., S. Neale, L. Paterson, A.C. Riches, K. Dholakia & G.C. Spalding. 2004. Cell cytometry with a light touch: Sorting microscopic matter with an optical lattice. Journal of Biological Regulators and Homeostatic Agents 18: 200-205. [abstract] [full text] Lake, T.K., A.E. Carruthers, L. Paterson, M. Taylor, F. Gunn-Moore, J.W. Allen, W. Sibbett & K. Dholakia. 2004. Optical trapping and fluorescence excitation with violet diode lasers and extended cavity surface emitting lasers. Optics Express 12: 670-678. [link] Dholakia, K., H. Little, C.T.A. Brown, B. Agate, D. McGloin, L. Paterson & W. Sibbett. 2004. Imaging in optical micromanipulation using two-photon excitation. New Journal of Physics 6: 136-143. [abstract] [full text] 

2003

Paterson, L., M.P. MacDonald, J. Arlt, W. Dultz, H. Schmitzer, W. Sibbett & K. Dholakia. 2003. Controlled simultaneous rotation of multiple optically trapped particles. Journal of Modern Optics 50: 1591-1599. [link]

2002

MacDonald, M.P., K. Volke-Sepulveda, L. Paterson, J. Arlt, W. Sibbett & K. Dholakia. 2002. Revolving interference patterns for the rotation of optically trapped particles. Optics Communications 201: 21-28. [link] MacDonald, M.P., L. Paterson, K. Volke-Sepulveda, J. Arlt, W. Sibbett & K. Dholakia. 2002. Creation and manipulation of three-dimensional optically trapped structures. Science 296: 1101-1103. [abstract] [full text (limited access)] Arlt, J., M. MacDonald, L. Paterson, W. Sibbett, K. Dholakia & K. Volke-Sepulveda. 2002. Moving interference patterns created using the angular Doppler- effect. Optics Express 10: 844-852. [link] 

2001

Paterson, L., M.P. MacDonald, J. Arlt, W. Sibbett, P.E. Bryant & K. Dholakia. 2001. Controlled rotation of optically trapped microscopic particles. Science 292: 912-914. [abstract] [full text (limited access)] MacDonald, M.P., L. Paterson, W. Sibbett, K. Dholakia & P.E. Bryant. 2001. Trapping and manipulation of low-index particles in a two- dimensional interferometric optical trap. Optics Letters 26: 863-865. [link]